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e coli atcc 15597  (ATCC)


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    Structured Review

    ATCC e coli atcc 15597
    Summary of key studies on phage-nanoparticle synergy.
    E Coli Atcc 15597, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 770 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+atcc+15597/Escherichia+coli/pmc12975878-11-8-10
    Average 97 stars, based on 770 article reviews
    e coli atcc 15597 - by Bioz Stars, 2026-09
    97/100 stars

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    1) Product Images from "Searching for the perfect match: can non-antibiotic antimicrobials improve bacteriophage performance?"

    Article Title: Searching for the perfect match: can non-antibiotic antimicrobials improve bacteriophage performance?

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2026.1758422


    Figure Legend Snippet: Summary of key studies on phage-nanoparticle synergy.

    Techniques Used: Concentration Assay, Binding Assay, Inhibition, Crystal Violet Assay, Growth Inhibition Assay, Activity Assay

    Related Articles

    Incubation:

    Article Title: Surface modification of commercial anion exchange membrane for the inactivation of Escherichia coli.
    Article Snippet: .. In detail, E. coli ATCC 15597 was inoculated into sterilized liquid TSB (pH 7.3) and incubated overnight at 37°C. ..

    Article Title: Physicochemical, genomic, and phenotypic characterization of Escherichia phage BME3
    Article Snippet: .. The samples were incubated at 37°C for 1 h. To determine the titer of each treatment, the double-layer agar method was employed using E. coli ATCC 15597 as the host strain. ..

    Article Title: Physicochemical, genomic, and phenotypic characterization of Escherichia phage BME3
    Article Snippet: .. Then, 10 mL of logarithmic phase-growing E. coli ATCC 15597 (0.5 McFarland standard) was added to the mixture and incubated for 5 min at 37°C. ..

    Article Title: Searching for the perfect match: can non-antibiotic antimicrobials improve bacteriophage performance?
    Article Snippet: .. MS2 + T7 phages , Cinnamon Thymol , E. coli ATCC 15597 , Liquid culture , Time-kill curve assay at 37 °C after exposure to 1⁄2 MIC of EO and phage (MOI = 0.1) , Lytic activity of the cocktail significantly enhanced in the presence of all EOs after 24-h incubation at 37 °C , , , Less phage resistant mutants (6-8%) than in phage alone samples (88%) , ( ) . ..

    other:

    Article Title: Physicochemical, genomic, and phenotypic characterization of Escherichia phage BME3
    Article Snippet: Phage titer was determined by the double-layer agar method, and E. coli ATCC 15597 was used as host.

    Article Title: Synergistic Approaches to Foodborne Pathogen Control: A Narrative Review of Essential Oils and Bacteriophages.
    Article Snippet: Finally, Elafify et al. [93] tested multiple EOs (cinnamon, thymol, clove) alongside a phage cocktail (MS2 + T7) against E. coli ATCC 15597.

    Activity Assay:

    Article Title: Searching for the perfect match: can non-antibiotic antimicrobials improve bacteriophage performance?
    Article Snippet: .. MS2 + T7 phages , Cinnamon Thymol , E. coli ATCC 15597 , Liquid culture , Time-kill curve assay at 37 °C after exposure to 1⁄2 MIC of EO and phage (MOI = 0.1) , Lytic activity of the cocktail significantly enhanced in the presence of all EOs after 24-h incubation at 37 °C , , , Less phage resistant mutants (6-8%) than in phage alone samples (88%) , ( ) . ..

    In Vitro:

    Article Title: Synergistic Approaches to Foodborne Pathogen Control: A Narrative Review of Essential Oils and Bacteriophages
    Article Snippet: Kim et al., 2024 [ ] , S. typhimurium , Carvacrol, eugenol, thymol (1⁄2 MIC), AITC (allyl isothiocyanate) , Phage endolysin (LysPB32) , Cooked ground beef , In vitro + food trial , >2-log CFU/g reduction in meat; synergistic membrane disruption. .. Elafify et al., 2025 [ ] , E. coli ATCC 15597 , Cinnamon, thymol (1⁄2 MIC) , MS2 + T7 cocktail , In vitro , Spot test, time-kill assay, fitness/mutation assays , Strong synergy; >5-log reduction; reduced resistance. ..

    Spot Test:

    Article Title: Synergistic Approaches to Foodborne Pathogen Control: A Narrative Review of Essential Oils and Bacteriophages
    Article Snippet: Kim et al., 2024 [ ] , S. typhimurium , Carvacrol, eugenol, thymol (1⁄2 MIC), AITC (allyl isothiocyanate) , Phage endolysin (LysPB32) , Cooked ground beef , In vitro + food trial , >2-log CFU/g reduction in meat; synergistic membrane disruption. .. Elafify et al., 2025 [ ] , E. coli ATCC 15597 , Cinnamon, thymol (1⁄2 MIC) , MS2 + T7 cocktail , In vitro , Spot test, time-kill assay, fitness/mutation assays , Strong synergy; >5-log reduction; reduced resistance. ..

    Time-Kill Assay:

    Article Title: Synergistic Approaches to Foodborne Pathogen Control: A Narrative Review of Essential Oils and Bacteriophages
    Article Snippet: Kim et al., 2024 [ ] , S. typhimurium , Carvacrol, eugenol, thymol (1⁄2 MIC), AITC (allyl isothiocyanate) , Phage endolysin (LysPB32) , Cooked ground beef , In vitro + food trial , >2-log CFU/g reduction in meat; synergistic membrane disruption. .. Elafify et al., 2025 [ ] , E. coli ATCC 15597 , Cinnamon, thymol (1⁄2 MIC) , MS2 + T7 cocktail , In vitro , Spot test, time-kill assay, fitness/mutation assays , Strong synergy; >5-log reduction; reduced resistance. ..



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    Image Search Results


    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Searching for the perfect match: can non-antibiotic antimicrobials improve bacteriophage performance?

    doi: 10.3389/fcimb.2026.1758422

    Figure Lengend Snippet: Summary of key studies on phage-nanoparticle synergy.

    Article Snippet: MS2 + T7 phages , Cinnamon Thymol , E. coli ATCC 15597 , Liquid culture , Time-kill curve assay at 37 °C after exposure to 1⁄2 MIC of EO and phage (MOI = 0.1) , Lytic activity of the cocktail significantly enhanced in the presence of all EOs after 24-h incubation at 37 °C , , , Less phage resistant mutants (6-8%) than in phage alone samples (88%) , ( ) .

    Techniques: Concentration Assay, Binding Assay, Inhibition, Crystal Violet Assay, Growth Inhibition Assay, Activity Assay

    Enumeration of S. Saintpaul B23 as a donor (DR), E. coli ATCC15597 (RT), and the transconjugants (TC) in hydroponic solution ( A ), in plant growth substrates ( B ), and on hydroponic facility surfaces ( C ) in the conjugation assays. Each column represents the average of triplicates, and each error bar indicates the standard deviation. * denotes a significant difference between the results in hydroponic solution (HS) and 10% broth ( p < 0.05 by unpaired t test); N.S. denotes no significance. ^ denotes values below the detection limit (1 log CFU/cm 2 ).

    Journal: Pathogens

    Article Title: Antimicrobial Resistance Transmission of Multidrug-Resistant Bacteria in Hydroponic Farming Components

    doi: 10.3390/pathogens14111134

    Figure Lengend Snippet: Enumeration of S. Saintpaul B23 as a donor (DR), E. coli ATCC15597 (RT), and the transconjugants (TC) in hydroponic solution ( A ), in plant growth substrates ( B ), and on hydroponic facility surfaces ( C ) in the conjugation assays. Each column represents the average of triplicates, and each error bar indicates the standard deviation. * denotes a significant difference between the results in hydroponic solution (HS) and 10% broth ( p < 0.05 by unpaired t test); N.S. denotes no significance. ^ denotes values below the detection limit (1 log CFU/cm 2 ).

    Article Snippet: The tested recipients included Salmonella Brunei HW5, a self-isolate from local hydroponic farm water in Singapore [ ]; E. coli ATCC15597; E. coli O157:H7 EDL 933; E. coli O157:H7 EDL 931, purchased from the American Type Culture Collection; and E. coli O157:H7 C7927, an apple cider isolate obtained from Dr. Kun-Ho Seo at Konkuk University, Republic of Korea [ ].

    Techniques: Conjugation Assay, Standard Deviation

    UV inactivation of S. Saintpaul B23 and E. coli ATCC15597. Each data point represents the average of triplicates, and each error bar indicates the standard deviation.

    Journal: Pathogens

    Article Title: Antimicrobial Resistance Transmission of Multidrug-Resistant Bacteria in Hydroponic Farming Components

    doi: 10.3390/pathogens14111134

    Figure Lengend Snippet: UV inactivation of S. Saintpaul B23 and E. coli ATCC15597. Each data point represents the average of triplicates, and each error bar indicates the standard deviation.

    Article Snippet: The tested recipients included Salmonella Brunei HW5, a self-isolate from local hydroponic farm water in Singapore [ ]; E. coli ATCC15597; E. coli O157:H7 EDL 933; E. coli O157:H7 EDL 931, purchased from the American Type Culture Collection; and E. coli O157:H7 C7927, an apple cider isolate obtained from Dr. Kun-Ho Seo at Konkuk University, Republic of Korea [ ].

    Techniques: Standard Deviation

    Enumeration of S. Saintpaul B23 as a donor (DR), E. coli ATCC15597 (RT), and the transconjugants (TC) in hydroponic solution after different doses of UV radiation ( A ) and after different antibiotic treatments at sub-MIC doses ( B ). TET denotes tetracycline, GEN denotes gentamicin, and CHL denotes chloramphenicol. Each column represents the average of triplicates, and each error bar indicates the standard deviation. * denotes a significant difference between the results in hydroponic solution (HS) and 10% broth ( p < 0.05 by one-way analysis of variance); N.S. denotes no significance.

    Journal: Pathogens

    Article Title: Antimicrobial Resistance Transmission of Multidrug-Resistant Bacteria in Hydroponic Farming Components

    doi: 10.3390/pathogens14111134

    Figure Lengend Snippet: Enumeration of S. Saintpaul B23 as a donor (DR), E. coli ATCC15597 (RT), and the transconjugants (TC) in hydroponic solution after different doses of UV radiation ( A ) and after different antibiotic treatments at sub-MIC doses ( B ). TET denotes tetracycline, GEN denotes gentamicin, and CHL denotes chloramphenicol. Each column represents the average of triplicates, and each error bar indicates the standard deviation. * denotes a significant difference between the results in hydroponic solution (HS) and 10% broth ( p < 0.05 by one-way analysis of variance); N.S. denotes no significance.

    Article Snippet: The tested recipients included Salmonella Brunei HW5, a self-isolate from local hydroponic farm water in Singapore [ ]; E. coli ATCC15597; E. coli O157:H7 EDL 933; E. coli O157:H7 EDL 931, purchased from the American Type Culture Collection; and E. coli O157:H7 C7927, an apple cider isolate obtained from Dr. Kun-Ho Seo at Konkuk University, Republic of Korea [ ].

    Techniques: Standard Deviation

    Characterization of bacteriophage BME3. ( A ) Plaque morphology of BME3 formed against E. coli ATCC 15597 determined by double-layer agar method. BME3 produces clear plaques with a diameter of less than 1 mm. ( B ) Transmission electron microscopy (TEM). Morphology of BME3 with a scale bar (200 nm). The phage virion was stained with 2% phosphotungstic acid and observed by TEM.

    Journal: Microbiology Spectrum

    Article Title: Physicochemical, genomic, and phenotypic characterization of Escherichia phage BME3

    doi: 10.1128/spectrum.01301-24

    Figure Lengend Snippet: Characterization of bacteriophage BME3. ( A ) Plaque morphology of BME3 formed against E. coli ATCC 15597 determined by double-layer agar method. BME3 produces clear plaques with a diameter of less than 1 mm. ( B ) Transmission electron microscopy (TEM). Morphology of BME3 with a scale bar (200 nm). The phage virion was stained with 2% phosphotungstic acid and observed by TEM.

    Article Snippet: The E. coli ATCC 15597 strain was used for the isolation and propagation of bacteriophages.

    Techniques: Transmission Assay, Electron Microscopy, Staining

    Characterization of bacteriophage BME3. ( A ) Plaque morphology of BME3 formed against E. coli ATCC 15597 determined by double-layer agar method. BME3 produces clear plaques with a diameter of less than 1 mm. ( B ) Transmission electron microscopy (TEM). Morphology of BME3 with a scale bar (200 nm). The phage virion was stained with 2% phosphotungstic acid and observed by TEM.

    Journal: Microbiology Spectrum

    Article Title: Physicochemical, genomic, and phenotypic characterization of Escherichia phage BME3

    doi: 10.1128/spectrum.01301-24

    Figure Lengend Snippet: Characterization of bacteriophage BME3. ( A ) Plaque morphology of BME3 formed against E. coli ATCC 15597 determined by double-layer agar method. BME3 produces clear plaques with a diameter of less than 1 mm. ( B ) Transmission electron microscopy (TEM). Morphology of BME3 with a scale bar (200 nm). The phage virion was stained with 2% phosphotungstic acid and observed by TEM.

    Article Snippet: BME3 was isolated from estuarine water sample, with a coliphage count of 58 PFU/100 mL, collected from Estero Salado using E. coli strain ATCC 15597, which is known for its susceptibility to bacteriophages ( ).

    Techniques: Transmission Assay, Electron Microscopy, Staining